Construct: shRNA TRCN0000033341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004422.1-454s1c1
DNA Barcode:
GAGACAGAAACCGAGTCAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DVL2 (1856)
Gene Description:
dishevelled segment polarity protein 2
Transcript:
RefSeq NM_004422.1 (NON-CURRENT)
Match location:
Position 454 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACAGAAACCGAGTCAGTA
Hairpin Sequence:
5'-CCGG-GAGACAGAAACCGAGTCAGTA-CTCGAG-TACTGACTCGGTTTCTGTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACAGAAACCGAGTCAGTACTCGAGTACTGACTCGGTTTCTGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACAGAAACCGAGTCAGTACTCGAGTACTGACTCGGTTTCTGTCTC-3'

Other clones with same target sequence:

  1. TRCN0000300447