Construct: shRNA TRCN0000033380

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030753.3-296s1c1
DNA Barcode:
CCGCAATTACATCGAGATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WNT3 (7473)
Gene Description:
Wnt family member 3
Transcript:
RefSeq NM_030753.3 (NON-CURRENT)
Match location:
Position 296 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033380)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCAATTACATCGAGATCAT
Hairpin Sequence:
5'-CCGG-CCGCAATTACATCGAGATCAT-CTCGAG-ATGATCTCGATGTAATTGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCAATTACATCGAGATCATCTCGAGATGATCTCGATGTAATTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCAATTACATCGAGATCATCTCGAGATGATCTCGATGTAATTGCGG-3'

Other clones with same target sequence:

(none)