Construct: shRNA TRCN0000033420

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002226.3-1132s1c1
DNA Barcode:
CTGTGTGTAAACAAGGGTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JAG2 (3714)
Gene Description:
jagged canonical Notch ligand 2
Transcript:
RefSeq NM_002226.3 (NON-CURRENT)
Match location:
Position 1132 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033420)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGTGTAAACAAGGGTGTA
Hairpin Sequence:
5'-CCGG-CTGTGTGTAAACAAGGGTGTA-CTCGAG-TACACCCTTGTTTACACACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGTGTAAACAAGGGTGTACTCGAGTACACCCTTGTTTACACACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGTGTAAACAAGGGTGTACTCGAGTACACCCTTGTTTACACACAG-3'

Other clones with same target sequence:

(none)