Construct: shRNA TRCN0000033446
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_005338.4-1117s1c1
- DNA Barcode:
- CCTTCAATTTCAACAGTCAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HIP1 (3092)
- Gene Description:
- huntingtin interacting protein 1
- Transcript:
- RefSeq NM_005338.4 (NON-CURRENT)
- Match location:
- Position 1117 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000033446)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTTCAATTTCAACAGTCAAA
- Hairpin Sequence:
-
5'-CCGG-CCTTCAATTTCAACAGTCAAA-CTCGAG-TTTGACTGTTGAAATTGAAGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTTCAATTTCAACAGTCAAACTCGAGTTTGACTGTTGAAATTGAAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTTCAATTTCAACAGTCAAACTCGAGTTTGACTGTTGAAATTGAAGG-3'
Other clones with same target sequence:
-
TRCN0000299591
