Construct: shRNA TRCN0000033451

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018014.2-547s1c1
DNA Barcode:
CCAGAGGATGACGATTGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCL11A (53335)
Gene Description:
BAF chromatin remodeling complex subunit BCL11A
Transcript:
RefSeq NM_018014.2 (NON-CURRENT)
Match location:
Position 547 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033451)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGGATGACGATTGTTTA
Hairpin Sequence:
5'-CCGG-CCAGAGGATGACGATTGTTTA-CTCGAG-TAAACAATCGTCATCCTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGGATGACGATTGTTTACTCGAGTAAACAATCGTCATCCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGGATGACGATTGTTTACTCGAGTAAACAATCGTCATCCTCTGG-3'

Other clones with same target sequence:

(none)