Construct: shRNA TRCN0000033575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001707.2-324s1c1
DNA Barcode:
CCTAATGGCTTTCCTTCTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCL7B (9275)
Gene Description:
BAF chromatin remodeling complex subunit BCL7B
Transcript:
RefSeq NM_001707.2 (NON-CURRENT)
Match location:
Position 324 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAATGGCTTTCCTTCTGAT
Hairpin Sequence:
5'-CCGG-CCTAATGGCTTTCCTTCTGAT-CTCGAG-ATCAGAAGGAAAGCCATTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAATGGCTTTCCTTCTGATCTCGAGATCAGAAGGAAAGCCATTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAATGGCTTTCCTTCTGATCTCGAGATCAGAAGGAAAGCCATTAGG-3'

Other clones with same target sequence:

  1. TRCN0000299792