Construct: shRNA TRCN0000033589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004282.2-719s1c1
DNA Barcode:
CCATCAAGCTATTAGAGCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BAG2 (9532)
Gene Description:
BAG cochaperone 2
Transcript:
RefSeq NM_004282.2 (NON-CURRENT)
Match location:
Position 719 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCAAGCTATTAGAGCATT
Hairpin Sequence:
5'-CCGG-CCATCAAGCTATTAGAGCATT-CTCGAG-AATGCTCTAATAGCTTGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCAAGCTATTAGAGCATTCTCGAGAATGCTCTAATAGCTTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCAAGCTATTAGAGCATTCTCGAGAATGCTCTAATAGCTTGATGG-3'

Other clones with same target sequence:

  1. TRCN0000299786