Construct: shRNA TRCN0000033607

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015678.2-6511s1c1
DNA Barcode:
GCACGGAAGAAGATGTAGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NBEA (26960)
Gene Description:
neurobeachin
Transcript:
RefSeq NM_015678.2 (NON-CURRENT)
Match location:
Position 6511 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033607)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACGGAAGAAGATGTAGTAA
Hairpin Sequence:
5'-CCGG-GCACGGAAGAAGATGTAGTAA-CTCGAG-TTACTACATCTTCTTCCGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACGGAAGAAGATGTAGTAACTCGAGTTACTACATCTTCTTCCGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACGGAAGAAGATGTAGTAACTCGAGTTACTACATCTTCTTCCGTGC-3'

Other clones with same target sequence:

(none)