Construct: shRNA TRCN0000033692

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021026.1-384s1c1
DNA Barcode:
GTCTGCTTCAAGTGCATCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RFPL1 (5988)
Gene Description:
ret finger protein like 1
Transcript:
RefSeq NM_021026.1 (NON-CURRENT)
Match location:
Position 384 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033692)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGCTTCAAGTGCATCAAT
Hairpin Sequence:
5'-CCGG-GTCTGCTTCAAGTGCATCAAT-CTCGAG-ATTGATGCACTTGAAGCAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGCTTCAAGTGCATCAATCTCGAGATTGATGCACTTGAAGCAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGCTTCAAGTGCATCAATCTCGAGATTGATGCACTTGAAGCAGAC-3'

Other clones with same target sequence:

(none)