Construct: shRNA TRCN0000033703

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005977.2-703s1c1
DNA Barcode:
GCGTCAAGGAACAACTAGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF6 (6049)
Gene Description:
ring finger protein 6
Transcript:
RefSeq NM_005977.2 (NON-CURRENT)
Match location:
Position 703 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033703)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCAAGGAACAACTAGCAT
Hairpin Sequence:
5'-CCGG-GCGTCAAGGAACAACTAGCAT-CTCGAG-ATGCTAGTTGTTCCTTGACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCAAGGAACAACTAGCATCTCGAGATGCTAGTTGTTCCTTGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCAAGGAACAACTAGCATCTCGAGATGCTAGTTGTTCCTTGACGC-3'

Other clones with same target sequence:

  1. TRCN0000333720