Construct: shRNA TRCN0000033758

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006604.1-565s1c1
DNA Barcode:
GCGGAAGTTCCAAGTGGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RFPL3 (10738)
Gene Description:
ret finger protein like 3
Transcript:
RefSeq NM_006604.1 (NON-CURRENT)
Match location:
Position 565 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033758)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAAGTTCCAAGTGGATAT
Hairpin Sequence:
5'-CCGG-GCGGAAGTTCCAAGTGGATAT-CTCGAG-ATATCCACTTGGAACTTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAAGTTCCAAGTGGATATCTCGAGATATCCACTTGGAACTTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAAGTTCCAAGTGGATATCTCGAGATATCCACTTGGAACTTCCGC-3'

Other clones with same target sequence:

(none)