Construct: shRNA TRCN0000033785

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015144.1-2744s1c1
DNA Barcode:
CGGGAACCTATCTTGTTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZCCHC14 (23174)
Gene Description:
zinc finger CCHC-type containing 14
Transcript:
RefSeq NM_015144.1 (NON-CURRENT)
Match location:
Position 2744 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033785)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAACCTATCTTGTTACAA
Hairpin Sequence:
5'-CCGG-CGGGAACCTATCTTGTTACAA-CTCGAG-TTGTAACAAGATAGGTTCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAACCTATCTTGTTACAACTCGAGTTGTAACAAGATAGGTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAACCTATCTTGTTACAACTCGAGTTGTAACAAGATAGGTTCCCG-3'

Other clones with same target sequence:

  1. TRCN0000291853