Construct: shRNA TRCN0000033819

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013262.3-456s1c1
DNA Barcode:
GCTTAAACTTAGAGTCAAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYLIP (29116)
Gene Description:
myosin regulatory light chain interacting protein
Transcript:
RefSeq NM_013262.3 (NON-CURRENT)
Match location:
Position 456 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033819)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAAACTTAGAGTCAAGTT
Hairpin Sequence:
5'-CCGG-GCTTAAACTTAGAGTCAAGTT-CTCGAG-AACTTGACTCTAAGTTTAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAAACTTAGAGTCAAGTTCTCGAGAACTTGACTCTAAGTTTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAAACTTAGAGTCAAGTTCTCGAGAACTTGACTCTAAGTTTAAGC-3'

Other clones with same target sequence:

(none)