Construct: shRNA TRCN0000033891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032546.2-614s1c1
DNA Barcode:
CCTCGCAGAACACTAAGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM54 (57159)
Gene Description:
tripartite motif containing 54
Transcript:
RefSeq NM_032546.2 (NON-CURRENT)
Match location:
Position 614 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGCAGAACACTAAGATTA
Hairpin Sequence:
5'-CCGG-CCTCGCAGAACACTAAGATTA-CTCGAG-TAATCTTAGTGTTCTGCGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGCAGAACACTAAGATTACTCGAGTAATCTTAGTGTTCTGCGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGCAGAACACTAAGATTACTCGAGTAATCTTAGTGTTCTGCGAGG-3'

Other clones with same target sequence:

(none)