Construct: shRNA TRCN0000033917

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023076.2-1162s1c1
DNA Barcode:
CCTGTCAGCACCACATCCTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C16orf28 (65259)
Gene Description:
chromosome 16 open reading frame 28
Transcript:
RefSeq NM_023076.2 (NON-CURRENT)
Match location:
Position 1162 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000033917)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCAGCACCACATCCTCT
Hairpin Sequence:
5'-CCGG-CCTGTCAGCACCACATCCTCT-CTCGAG-AGAGGATGTGGTGCTGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCAGCACCACATCCTCTCTCGAGAGAGGATGTGGTGCTGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCAGCACCACATCCTCTCTCGAGAGAGGATGTGGTGCTGACAGG-3'

Other clones with same target sequence:

(none)