Construct: shRNA TRCN0000034054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152267.2-1827s1c1
DNA Barcode:
GCTTGATAGAGTTCCCTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF185 (91445)
Gene Description:
ring finger protein 185
Transcript:
RefSeq NM_152267.2 (NON-CURRENT)
Match location:
Position 1827 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGATAGAGTTCCCTGAAA
Hairpin Sequence:
5'-CCGG-GCTTGATAGAGTTCCCTGAAA-CTCGAG-TTTCAGGGAACTCTATCAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGATAGAGTTCCCTGAAACTCGAGTTTCAGGGAACTCTATCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGATAGAGTTCCCTGAAACTCGAGTTTCAGGGAACTCTATCAAGC-3'

Other clones with same target sequence:

(none)