Construct: shRNA TRCN0000034075

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_057178.2-1175s1c1
DNA Barcode:
CCACATGGTAACCTGTACCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RFFL (117584)
Gene Description:
ring finger and FYVE like domain containing E3 ubiquitin protein ligase
Transcript:
RefSeq NM_057178.2 (NON-CURRENT)
Match location:
Position 1175 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034075)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACATGGTAACCTGTACCAA
Hairpin Sequence:
5'-CCGG-CCACATGGTAACCTGTACCAA-CTCGAG-TTGGTACAGGTTACCATGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACATGGTAACCTGTACCAACTCGAGTTGGTACAGGTTACCATGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACATGGTAACCTGTACCAACTCGAGTTGGTACAGGTTACCATGTGG-3'

Other clones with same target sequence:

(none)