Construct: shRNA TRCN0000034080

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_058166.2-1090s1c1
DNA Barcode:
GTGAGGTTTGTGGGAGCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM6 (117854)
Gene Description:
tripartite motif containing 6
Transcript:
RefSeq NM_058166.2 (NON-CURRENT)
Match location:
Position 1090 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034080)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAGGTTTGTGGGAGCTAAA
Hairpin Sequence:
5'-CCGG-GTGAGGTTTGTGGGAGCTAAA-CTCGAG-TTTAGCTCCCACAAACCTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAGGTTTGTGGGAGCTAAACTCGAGTTTAGCTCCCACAAACCTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAGGTTTGTGGGAGCTAAACTCGAGTTTAGCTCCCACAAACCTCAC-3'

Other clones with same target sequence:

  1. TRCN0000300453