Construct: shRNA TRCN0000034089

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153341.1-1579s1c1
DNA Barcode:
CGAGGGACAAACTGTCTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF19B (127544)
Gene Description:
ring finger protein 19B
Transcript:
RefSeq NM_153341.1 (NON-CURRENT)
Match location:
Position 1579 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034089)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGGACAAACTGTCTTGAA
Hairpin Sequence:
5'-CCGG-CGAGGGACAAACTGTCTTGAA-CTCGAG-TTCAAGACAGTTTGTCCCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGGACAAACTGTCTTGAACTCGAGTTCAAGACAGTTTGTCCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGGACAAACTGTCTTGAACTCGAGTTCAAGACAGTTTGTCCCTCG-3'

Other clones with same target sequence:

(none)