Construct: shRNA TRCN0000034261

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006315.3-755s1c1
DNA Barcode:
GCAGACGACAGTTCAAACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCGF3 (10336)
Gene Description:
polycomb group ring finger 3
Transcript:
RefSeq NM_006315.3 (NON-CURRENT)
Match location:
Position 755 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034261)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGACGACAGTTCAAACAAA
Hairpin Sequence:
5'-CCGG-GCAGACGACAGTTCAAACAAA-CTCGAG-TTTGTTTGAACTGTCGTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGACGACAGTTCAAACAAACTCGAGTTTGTTTGAACTGTCGTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGACGACAGTTCAAACAAACTCGAGTTTGTTTGAACTGTCGTCTGC-3'

Other clones with same target sequence:

(none)