Construct: shRNA TRCN0000034282

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013448.2-2989s1c1
DNA Barcode:
CGACCGCATGTATAGACGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BAZ1A (11177)
Gene Description:
bromodomain adjacent to zinc finger domain 1A
Transcript:
RefSeq NM_013448.2 (NON-CURRENT)
Match location:
Position 2989 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034282)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACCGCATGTATAGACGATA
Hairpin Sequence:
5'-CCGG-CGACCGCATGTATAGACGATA-CTCGAG-TATCGTCTATACATGCGGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACCGCATGTATAGACGATACTCGAGTATCGTCTATACATGCGGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACCGCATGTATAGACGATACTCGAGTATCGTCTATACATGCGGTCG-3'

Other clones with same target sequence:

(none)