Construct: shRNA TRCN0000034309

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012347.2-424s1c1
DNA Barcode:
CCTGATATAGAGTTCAAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXO9 (26268)
Gene Description:
F-box protein 9
Transcript:
RefSeq NM_012347.2 (NON-CURRENT)
Match location:
Position 424 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034309)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATATAGAGTTCAAGATT
Hairpin Sequence:
5'-CCGG-CCTGATATAGAGTTCAAGATT-CTCGAG-AATCTTGAACTCTATATCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATATAGAGTTCAAGATTCTCGAGAATCTTGAACTCTATATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATATAGAGTTCAAGATTCTCGAGAATCTTGAACTCTATATCAGG-3'

Other clones with same target sequence:

(none)