Construct: shRNA TRCN0000034339

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010897.1-11382s1c1
DNA Barcode:
CCCTGTAAATAGTTTGTGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nf1 (18015)
Gene Description:
neurofibromin 1
Transcript:
RefSeq NM_010897.1 (NON-CURRENT)
Match location:
Position 11382 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034339)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTAAATAGTTTGTGTAA
Hairpin Sequence:
5'-CCGG-CCCTGTAAATAGTTTGTGTAA-CTCGAG-TTACACAAACTATTTACAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTAAATAGTTTGTGTAACTCGAGTTACACAAACTATTTACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTAAATAGTTTGTGTAACTCGAGTTACACAAACTATTTACAGGG-3'

Other clones with same target sequence:

(none)