Construct: shRNA TRCN0000034388

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021284.2-689s1c1
DNA Barcode:
GATGAGCAAAGATGGGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kras (16653)
Gene Description:
Kirsten rat sarcoma viral oncogene homolog
Transcript:
RefSeq NM_021284.2 (NON-CURRENT)
Match location:
Position 689 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034388)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGCAAAGATGGGAAGAA
Hairpin Sequence:
5'-CCGG-GATGAGCAAAGATGGGAAGAA-CTCGAG-TTCTTCCCATCTTTGCTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGCAAAGATGGGAAGAACTCGAGTTCTTCCCATCTTTGCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGCAAAGATGGGAAGAACTCGAGTTCTTCCCATCTTTGCTCATC-3'

Other clones with same target sequence:

  1. TRCN0000301688