Construct: shRNA TRCN0000034394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008583.1-2762s1c1
DNA Barcode:
GCTCCGACTCTTATCTGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Men1 (17283)
Gene Description:
multiple endocrine neoplasia 1
Transcript:
RefSeq NM_008583.1 (NON-CURRENT)
Match location:
Position 2762 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCGACTCTTATCTGTGAA
Hairpin Sequence:
5'-CCGG-GCTCCGACTCTTATCTGTGAA-CTCGAG-TTCACAGATAAGAGTCGGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCGACTCTTATCTGTGAACTCGAGTTCACAGATAAGAGTCGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCGACTCTTATCTGTGAACTCGAGTTCACAGATAAGAGTCGGAGC-3'

Other clones with same target sequence:

(none)