Construct: shRNA TRCN0000034485

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011356.2-1171s1c1
DNA Barcode:
GAGGTAAAGATGAAATGTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Frzb (20378)
Gene Description:
frizzled-related protein
Transcript:
RefSeq NM_011356.2 (NON-CURRENT)
Match location:
Position 1171 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034485)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGTAAAGATGAAATGTCAT
Hairpin Sequence:
5'-CCGG-GAGGTAAAGATGAAATGTCAT-CTCGAG-ATGACATTTCATCTTTACCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGTAAAGATGAAATGTCATCTCGAGATGACATTTCATCTTTACCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGTAAAGATGAAATGTCATCTCGAGATGACATTTCATCTTTACCTC-3'

Other clones with same target sequence:

(none)