Construct: shRNA TRCN0000034496

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019972.2-1626s1c1
DNA Barcode:
CCGTCCTATCAATGTGATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sort1 (20661)
Gene Description:
sortilin 1
Transcript:
RefSeq NM_019972.2 (NON-CURRENT)
Match location:
Position 1626 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034496)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTCCTATCAATGTGATTAA
Hairpin Sequence:
5'-CCGG-CCGTCCTATCAATGTGATTAA-CTCGAG-TTAATCACATTGATAGGACGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTCCTATCAATGTGATTAACTCGAGTTAATCACATTGATAGGACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTCCTATCAATGTGATTAACTCGAGTTAATCACATTGATAGGACGG-3'

Other clones with same target sequence:

  1. TRCN0000301697