Construct: shRNA TRCN0000034503

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011465.2-2191s1c1
DNA Barcode:
GCAGGTTACCTCTGAGGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spta1 (20739)
Gene Description:
spectrin alpha, erythrocytic 1
Transcript:
RefSeq NM_011465.2 (NON-CURRENT)
Match location:
Position 2191 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034503)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGTTACCTCTGAGGATTA
Hairpin Sequence:
5'-CCGG-GCAGGTTACCTCTGAGGATTA-CTCGAG-TAATCCTCAGAGGTAACCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGTTACCTCTGAGGATTACTCGAGTAATCCTCAGAGGTAACCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGTTACCTCTGAGGATTACTCGAGTAATCCTCAGAGGTAACCTGC-3'

Other clones with same target sequence:

(none)