Construct: shRNA TRCN0000034514

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_290331.4-2143s1c1
DNA Barcode:
CCCAGCATCACCAATGAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GGT3P (2679)
Gene Description:
gamma-glutamyltransferase 3 pseudogene
Transcript:
RefSeq XM_290331.4 (NON-CURRENT)
Match location:
Position 2143 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034514)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCATCACCAATGAGTTT
Hairpin Sequence:
5'-CCGG-CCCAGCATCACCAATGAGTTT-CTCGAG-AAACTCATTGGTGATGCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCATCACCAATGAGTTTCTCGAGAAACTCATTGGTGATGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCATCACCAATGAGTTTCTCGAGAAACTCATTGGTGATGCTGGG-3'

Other clones with same target sequence:

(none)