Construct: shRNA TRCN0000034539

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019077.2-679s1c1
DNA Barcode:
GTCTTAGAAATAGCCTCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UGT1A7 (54577)
Gene Description:
UDP glucuronosyltransferase family 1 member A7
Transcript:
RefSeq NM_019077.2 (CURRENT)
Match location:
Position 679 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034539)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTTAGAAATAGCCTCTGAA
Hairpin Sequence:
5'-CCGG-GTCTTAGAAATAGCCTCTGAA-CTCGAG-TTCAGAGGCTATTTCTAAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTTAGAAATAGCCTCTGAACTCGAGTTCAGAGGCTATTTCTAAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTTAGAAATAGCCTCTGAACTCGAGTTCAGAGGCTATTTCTAAGAC-3'

Other clones with same target sequence:

(none)