Construct: shRNA TRCN0000034556

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001303.2-519s1c1
DNA Barcode:
CAGCCTATCTTTGTCCAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COX10 (1352)
Gene Description:
cytochrome c oxidase assembly factor heme A:farnesyltransferase COX10
Transcript:
RefSeq NM_001303.2 (NON-CURRENT)
Match location:
Position 519 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034556)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCCTATCTTTGTCCAGAAA
Hairpin Sequence:
5'-CCGG-CAGCCTATCTTTGTCCAGAAA-CTCGAG-TTTCTGGACAAAGATAGGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCCTATCTTTGTCCAGAAACTCGAGTTTCTGGACAAAGATAGGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCCTATCTTTGTCCAGAAACTCGAGTTTCTGGACAAAGATAGGCTG-3'

Other clones with same target sequence:

(none)