Construct: shRNA TRCN0000034617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019093.2-856s1c1
DNA Barcode:
CCACTATCTCAGGAATTTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UGT1A3 (54659)
Gene Description:
UDP glucuronosyltransferase family 1 member A3
Transcript:
RefSeq NM_019093.2 (CURRENT)
Match location:
Position 856 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTATCTCAGGAATTTGAA
Hairpin Sequence:
5'-CCGG-CCACTATCTCAGGAATTTGAA-CTCGAG-TTCAAATTCCTGAGATAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTATCTCAGGAATTTGAACTCGAGTTCAAATTCCTGAGATAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTATCTCAGGAATTTGAACTCGAGTTCAAATTCCTGAGATAGTGG-3'

Other clones with same target sequence:

(none)