Construct: shRNA TRCN0000034732

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053039.1-1377s1c1
DNA Barcode:
CGAGCAGTCTTCTGGATTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UGT2B28 (54490)
Gene Description:
UDP glucuronosyltransferase family 2 member B28
Transcript:
RefSeq NM_053039.1 (NON-CURRENT)
Match location:
Position 1377 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034732)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCAGTCTTCTGGATTGAA
Hairpin Sequence:
5'-CCGG-CGAGCAGTCTTCTGGATTGAA-CTCGAG-TTCAATCCAGAAGACTGCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCAGTCTTCTGGATTGAACTCGAGTTCAATCCAGAAGACTGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCAGTCTTCTGGATTGAACTCGAGTTCAATCCAGAAGACTGCTCG-3'

Other clones with same target sequence:

(none)