Construct: shRNA TRCN0000034759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004854.2-433s1c1
DNA Barcode:
CCAGATGTGTACAGTGCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHST10 (9486)
Gene Description:
carbohydrate sulfotransferase 10
Transcript:
RefSeq NM_004854.2 (NON-CURRENT)
Match location:
Position 433 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034759)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATGTGTACAGTGCCAAA
Hairpin Sequence:
5'-CCGG-CCAGATGTGTACAGTGCCAAA-CTCGAG-TTTGGCACTGTACACATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATGTGTACAGTGCCAAACTCGAGTTTGGCACTGTACACATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATGTGTACAGTGCCAAACTCGAGTTTGGCACTGTACACATCTGG-3'

Other clones with same target sequence:

(none)