Construct: shRNA TRCN0000034787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002079.1-1131s1c1
DNA Barcode:
GCAGGTTGAGTATCTGGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GOT1 (2805)
Gene Description:
glutamic-oxaloacetic transaminase 1
Transcript:
RefSeq NM_002079.1 (NON-CURRENT)
Match location:
Position 1131 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGTTGAGTATCTGGTCAA
Hairpin Sequence:
5'-CCGG-GCAGGTTGAGTATCTGGTCAA-CTCGAG-TTGACCAGATACTCAACCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGTTGAGTATCTGGTCAACTCGAGTTGACCAGATACTCAACCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGTTGAGTATCTGGTCAACTCGAGTTGACCAGATACTCAACCTGC-3'

Other clones with same target sequence:

(none)