Construct: shRNA TRCN0000034835

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005769.1-593s1c1
DNA Barcode:
TGTGACATCATCCCACAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHST4 (10164)
Gene Description:
carbohydrate sulfotransferase 4
Transcript:
RefSeq NM_005769.1 (NON-CURRENT)
Match location:
Position 593 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034835)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGACATCATCCCACAAGAT
Hairpin Sequence:
5'-CCGG-TGTGACATCATCCCACAAGAT-CTCGAG-ATCTTGTGGGATGATGTCACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGACATCATCCCACAAGATCTCGAGATCTTGTGGGATGATGTCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGACATCATCCCACAAGATCTCGAGATCTTGTGGGATGATGTCACA-3'

Other clones with same target sequence:

(none)