Construct: shRNA TRCN0000034925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000663.2-1431s1c1
DNA Barcode:
CCCGATGATTCCATACGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABAT (18)
Gene Description:
4-aminobutyrate aminotransferase
Transcript:
RefSeq NM_000663.2 (NON-CURRENT)
Match location:
Position 1431 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGATGATTCCATACGGAAT
Hairpin Sequence:
5'-CCGG-CCCGATGATTCCATACGGAAT-CTCGAG-ATTCCGTATGGAATCATCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGATGATTCCATACGGAATCTCGAGATTCCGTATGGAATCATCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGATGATTCCATACGGAATCTCGAGATTCCGTATGGAATCATCGGG-3'

Other clones with same target sequence:

  1. TRCN0000327875