Construct: shRNA TRCN0000034929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_031104.7-237s1c1
DNA Barcode:
CCTGGGCACTTTCTACTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GALNT16 (57452)
Gene Description:
polypeptide N-acetylgalactosaminyltransferase 16
Transcript:
RefSeq XM_031104.7 (NON-CURRENT)
Match location:
Position 237 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000034929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGGCACTTTCTACTACTT
Hairpin Sequence:
5'-CCGG-CCTGGGCACTTTCTACTACTT-CTCGAG-AAGTAGTAGAAAGTGCCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGGCACTTTCTACTACTTCTCGAGAAGTAGTAGAAAGTGCCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGGCACTTTCTACTACTTCTCGAGAAGTAGTAGAAAGTGCCCAGG-3'

Other clones with same target sequence:

(none)