Construct: shRNA TRCN0000035020

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194318.2-1311s1c1
DNA Barcode:
CGCCAGTAAATGTCGATGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
B3GLCT (145173)
Gene Description:
beta 3-glucosyltransferase
Transcript:
RefSeq NM_194318.2 (NON-CURRENT)
Match location:
Position 1311 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035020)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCAGTAAATGTCGATGCTA
Hairpin Sequence:
5'-CCGG-CGCCAGTAAATGTCGATGCTA-CTCGAG-TAGCATCGACATTTACTGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCAGTAAATGTCGATGCTACTCGAGTAGCATCGACATTTACTGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCAGTAAATGTCGATGCTACTCGAGTAGCATCGACATTTACTGGCG-3'

Other clones with same target sequence:

(none)