Construct: shRNA TRCN0000035036

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178537.3-1089s1c1
DNA Barcode:
GCAATTTGTGTACCTGTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
B4GALNT4 (338707)
Gene Description:
beta-1,4-N-acetyl-galactosaminyltransferase 4
Transcript:
RefSeq NM_178537.3 (NON-CURRENT)
Match location:
Position 1089 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035036)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAATTTGTGTACCTGTCCTT
Hairpin Sequence:
5'-CCGG-GCAATTTGTGTACCTGTCCTT-CTCGAG-AAGGACAGGTACACAAATTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAATTTGTGTACCTGTCCTTCTCGAGAAGGACAGGTACACAAATTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAATTTGTGTACCTGTCCTTCTCGAGAAGGACAGGTACACAAATTGC-3'

Other clones with same target sequence:

(none)