Construct: shRNA TRCN0000035164
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_293157.1-281s1c1
- DNA Barcode:
- CGTCCAATGATGCTCTGATAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LOC343705 (343705)
- Gene Description:
- similar to CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase (Beta-galactoside alpha-2,3-sialyltransferase) (Alpha 2,3-ST) (Gal-NAc6S) (Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase) (ST3GalIA) (ST3O) (ST3GalA.1) (SIAT4-A) (S...
- Transcript:
- RefSeq XM_293157.1 (NON-CURRENT)
- Match location:
- Position 281 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000035164)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGTCCAATGATGCTCTGATAT
- Hairpin Sequence:
-
5'-CCGG-CGTCCAATGATGCTCTGATAT-CTCGAG-ATATCAGAGCATCATTGGACG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGTCCAATGATGCTCTGATATCTCGAGATATCAGAGCATCATTGGACGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGTCCAATGATGCTCTGATATCTCGAGATATCAGAGCATCATTGGACG-3'
Other clones with same target sequence:
(none)
