Construct: shRNA TRCN0000035197
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_002406.2-1021s1c1
- DNA Barcode:
- CCACCGCAAGTTCCAGGGCTA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MGAT1 (4245)
- Gene Description:
- alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
- Transcript:
- RefSeq NM_002406.2 (NON-CURRENT)
- Match location:
- Position 1021 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000035197)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCACCGCAAGTTCCAGGGCTA
- Hairpin Sequence:
-
5'-CCGG-CCACCGCAAGTTCCAGGGCTA-CTCGAG-TAGCCCTGGAACTTGCGGTGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCACCGCAAGTTCCAGGGCTACTCGAGTAGCCCTGGAACTTGCGGTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCACCGCAAGTTCCAGGGCTACTCGAGTAGCCCTGGAACTTGCGGTGG-3'
Other clones with same target sequence:
(none)
