Construct: shRNA TRCN0000035200

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001054.2-439s1c1
DNA Barcode:
CAGAAGGTCAAGGTGGTCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SULT1A2 (6799)
Gene Description:
sulfotransferase family 1A member 2
Transcript:
RefSeq NM_001054.2 (NON-CURRENT)
Match location:
Position 439 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035200)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAAGGTCAAGGTGGTCTAT
Hairpin Sequence:
5'-CCGG-CAGAAGGTCAAGGTGGTCTAT-CTCGAG-ATAGACCACCTTGACCTTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAAGGTCAAGGTGGTCTATCTCGAGATAGACCACCTTGACCTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAAGGTCAAGGTGGTCTATCTCGAGATAGACCACCTTGACCTTCTG-3'

Other clones with same target sequence:

  1. TRCN0000181155