Construct: shRNA TRCN0000035209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005668.3-708s1c1
DNA Barcode:
CCAATGAAGAATCGCAGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST8SIA4 (7903)
Gene Description:
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4
Transcript:
RefSeq NM_005668.3 (NON-CURRENT)
Match location:
Position 708 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035209)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAATGAAGAATCGCAGGTTT
Hairpin Sequence:
5'-CCGG-CCAATGAAGAATCGCAGGTTT-CTCGAG-AAACCTGCGATTCTTCATTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAATGAAGAATCGCAGGTTTCTCGAGAAACCTGCGATTCTTCATTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAATGAAGAATCGCAGGTTTCTCGAGAAACCTGCGATTCTTCATTGG-3'

Other clones with same target sequence:

(none)