Construct: shRNA TRCN0000035258

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006011.2-220s1c1
DNA Barcode:
GCTGTTGTTGACAGAAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST8SIA2 (8128)
Gene Description:
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
Transcript:
RefSeq NM_006011.2 (NON-CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035258)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTGTTGACAGAAGTAAT
Hairpin Sequence:
5'-CCGG-GCTGTTGTTGACAGAAGTAAT-CTCGAG-ATTACTTCTGTCAACAACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTGTTGACAGAAGTAATCTCGAGATTACTTCTGTCAACAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTGTTGACAGAAGTAATCTCGAGATTACTTCTGTCAACAACAGC-3'

Other clones with same target sequence:

(none)