Construct: shRNA TRCN0000035266

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021154.3-122s1c1
DNA Barcode:
GCACTCAGTGTTGTTAGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSAT1 (29968)
Gene Description:
phosphoserine aminotransferase 1
Transcript:
RefSeq NM_021154.3 (NON-CURRENT)
Match location:
Position 122 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035266)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTCAGTGTTGTTAGAGAT
Hairpin Sequence:
5'-CCGG-GCACTCAGTGTTGTTAGAGAT-CTCGAG-ATCTCTAACAACACTGAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTCAGTGTTGTTAGAGATCTCGAGATCTCTAACAACACTGAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTCAGTGTTGTTAGAGATCTCGAGATCTCTAACAACACTGAGTGC-3'

Other clones with same target sequence:

  1. TRCN0000291729