Construct: shRNA TRCN0000035300

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013305.2-1510s1c1
DNA Barcode:
CCTCTACATCACTCACCACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ST8SIA5 (29906)
Gene Description:
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5
Transcript:
RefSeq NM_013305.2 (NON-CURRENT)
Match location:
Position 1510 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035300)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTACATCACTCACCACTA
Hairpin Sequence:
5'-CCGG-CCTCTACATCACTCACCACTA-CTCGAG-TAGTGGTGAGTGATGTAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTACATCACTCACCACTACTCGAGTAGTGGTGAGTGATGTAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTACATCACTCACCACTACTCGAGTAGTGGTGAGTGATGTAGAGG-3'

Other clones with same target sequence:

(none)