Construct: shRNA TRCN0000035316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371801.2-970s1c1
DNA Barcode:
CGACTTGCTATCAAAGTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MBOAT1 (154141)
Gene Description:
membrane bound O-acyltransferase domain containing 1
Transcript:
RefSeq XM_371801.2 (NON-CURRENT)
Match location:
Position 970 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACTTGCTATCAAAGTGAAA
Hairpin Sequence:
5'-CCGG-CGACTTGCTATCAAAGTGAAA-CTCGAG-TTTCACTTTGATAGCAAGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACTTGCTATCAAAGTGAAACTCGAGTTTCACTTTGATAGCAAGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACTTGCTATCAAAGTGAAACTCGAGTTTCACTTTGATAGCAAGTCG-3'

Other clones with same target sequence:

(none)