Construct: shRNA TRCN0000035323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004775.2-1138s1c1
DNA Barcode:
GAGTTCACTATGCTGGATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
B4GALT6 (9331)
Gene Description:
beta-1,4-galactosyltransferase 6
Transcript:
RefSeq NM_004775.2 (NON-CURRENT)
Match location:
Position 1138 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTCACTATGCTGGATATA
Hairpin Sequence:
5'-CCGG-GAGTTCACTATGCTGGATATA-CTCGAG-TATATCCAGCATAGTGAACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTCACTATGCTGGATATACTCGAGTATATCCAGCATAGTGAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTCACTATGCTGGATATACTCGAGTATATCCAGCATAGTGAACTC-3'

Other clones with same target sequence:

(none)