Construct: shRNA TRCN0000035414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003491.2-178s1c1
DNA Barcode:
CCCGAGAACTACCAGATGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAA10 (8260)
Gene Description:
N(alpha)-acetyltransferase 10, NatA catalytic subunit
Transcript:
RefSeq NM_003491.2 (NON-CURRENT)
Match location:
Position 178 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000035414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGAGAACTACCAGATGAAA
Hairpin Sequence:
5'-CCGG-CCCGAGAACTACCAGATGAAA-CTCGAG-TTTCATCTGGTAGTTCTCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGAGAACTACCAGATGAAACTCGAGTTTCATCTGGTAGTTCTCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGAGAACTACCAGATGAAACTCGAGTTTCATCTGGTAGTTCTCGGG-3'

Other clones with same target sequence:

  1. TRCN0000288748